The Demo Site

* Contributors
* PDF version of book portionPreface Seng-Lai Tan, Ph.D.
* PDF version of book portionIntroduction David R. Gretch
* PDF version of book portionChapter 1 HCV Genome and Life Cycle Stéphane Chevaliez and Jean-Michel Pawlotsky
o HCV Genome Organization and Function
o HCV Genome Structure and Organization
o Characteristics and Functions of HCV Proteins
o The HCV Lifecycle
o Structure of HCV Virions
o Conclusion
o References
* PDF version of book portionChapter 2 HCV 5′ and 3′UTR: When Translation Meets Replication Stephanie T. Shi and Michael M. C. Lai
o Introduction
o Structural and Functional Components of the HCV RNA
o HCV Translation
o HCV RNA Replication
o Regulation of HCV Translation and Replication
o Perspectives
o References
* PDF version of book portionChapter 3 Assemble and Interact: Pleiotropic Functions of the HCV Core Protein Stephen J. Polyak, Kevin C. Klein, Ikuo Shoji, Tatsuo Miyamura, and Jaisri R. Lingappa
o Introduction
o The Core of the Problem
o The Role of HCV Core in Capsid Assembly
o Sub-Cellular Targeting of HCV Core
o Post-Translational Modifications of HCV Core
o HCV Core-Host Interactions
o References
* PDF version of book portionChapter 4 HCV Glycoproteins: Assembly of a Functional E1–E2 Heterodimer Muriel Lavie, Anne Goffard, and Jean Dubuisson
o Introduction
o Biogenesis of HCV Envelope Glycoproteins
o Role of HCV Envelope Glycoproteins in Virus Entry
o Conclusion
o Future Trends
o References
* PDF version of book portionChapter 5 HCV NS2/3 Protease Sarah Welbourn and Arnim Pause
o Introduction
o NS2/3 Catalytic Cleavage
o Role of NS2/3 Cleavage in Viral Replication
o Roles of Cleaved NS2
o Conclusions
o References
* PDF version of book portionChapter 6 HCV NS3-4A Serine Protease Chao Lin
o Introduction
o Biological Functions
o Three Dimensional Structures
o Assays for the HCV NS3-4A Serine Protease
o A Drug Discovery Target
o Preclinical Studies and Clinical Development
o Conclusions and Future Directions
o References
* PDF version of book portionChapter 7 HCV Helicase: Structure, Function, and Inhibition David N. Frick
o Introduction
o Structure of HCV Helicase
o Mechanism of Action
o HCV Helicase Inhibitors
o Conclusions and Future Directions
o References
* PDF version of book portionChapter 8 HCV NS4B: From Obscurity to Central Stage Ella H. Sklan and Jeffrey S. Glenn
o Introduction
o Proteolytic Generation of NS4B
o Subcellular Localization and Topology
o NS4B and Lipid Rafts
o The Immunological Effects of NS4B
o Modulation of Ns5bs' RNA-Dependent RNA Polymerase Activity
o Effects of NS4B on Translation
o "Membranous Web" Formation
o Modulation of ER Functions
o Malignant Transformation
o NS4B Features That May Underlie the Mechanisms of the Above Functions
o Future Directions
o Future Directions
o References
* PDF version of book portionChapter 9 HCV NS5A: A Multifunctional Regulator of Cellular Pathways and Virus Replication Yupeng He, Kirk A. Staschke, and Seng-Lai Tan
o Introduction
o Structural Features and Subcellular Localization of NS5A
o NS5A Phosphorylation: A Functional Role or Red Herring?
o Emerging Role of NS5A in HCV Replication
o NS5A as a Viral Interceptor of Cellular Pathways
o Concluding Remarks
o References
* PDF version of book portionChapter 10 Biochemical Activities of the HCV NS5B RNA-Dependent RNA Polymerase C. T. Ranjith-Kumar and C. Cheng Kao
o Introduction
o Expression of NS5B
o Structural Features of HCV NS5B
o Catalytic Pocket
o Activities of NS5B
o De novo Synthesis and Primer Extension
o Mutational Analysis
o Terminal Nucleotidyl Transferase (TNTase) Activity
o Template Switch
o Interaction with Other HCV Nonstructural Proteins
o Conclusion and Future Trends
o References
* PDF version of book portionChapter 11 HCV Replicon Systems Keril J. Blight and Elizabeth A. Norgard
o Introduction
o Development of HCV Replicons
o Identification of Adaptive Mutations
o Generation of Replicons from Other HCV Isolates
o Development of Alternative Replicon Derivatives
o Cell Lines Permissive for HCV Replication
o Applications of the HCV Replicon System
o Inhibition of Replicon Replication
o Concluding Remarks
o References
* PDF version of book portionChapter 12 Animal Models for HCV Study Linda B. Couto and Alexander A. Kolykhalov
o Chimpanzee Model
o Transgenic Mouse Models
o Xenograft Models
o Small Non-Human Primate Models
o Summary and Conclusions
o References
* PDF version of book portionChapter 13 HCV Regulation of Host Defense D. Spencer Carney and Michael Gale Jr.
o Introduction
o Innate Intracellular Immune Defenses
o ISG Products Have Antiviral Functions
o Cross-Talk Between Innate and Adaptive Immune Defenses
o IFN-γ Activation of the Antiviral Response
o Hepatic Defenses Are Triggered by HCV
o Triggering the Host Response to HCV Infection
o Regulation and Evasion of the Host Response by HCV
o HCV Regulation of IFN Signaling
o Regulation of ISG Expression or Function
o Viral Genetics Impact the Host Response to HCV Infection
o Exogenous Induction of Antiviral Hepatic Defenses
o The HCV/Host Interface Model of Viral Evasion
o References
* PDF version of book portionChapter 14 Regulation of Adaptive Immunity by HCV Xiao-Song He
o Introduction
o Adaptive Immunity to HCV: A Failure in Most Patients
o Dendritic Cells–Are They Infected by HCV?
o Interference of Host Cell Functions by HCV
o Regulatory T Cells in HCV Infection
o Immune Cells and Immune Responses in the Liver
o Conclusion
o References
* PDF version of book portionChapter 15 Recombinant Vesicular Stomatitis Virus (VSV) and Other Strategies in HCV Vaccine Designs and Immunotherapy Ayaz M. Majid and Glen N. Barber
o Introduction
o Classical Strategies in Viral Vaccines
o Studies of Recombinant HCV Protein Sub-Units in Chimpanzees
o Efficacy of Recombinant DNA Approaches in Generating HCV Immunity
o Hepatitis C Virus-like Particles
o VSV as a Safe and Potent Vector for Generating Immunity against Viral Pathogens
o VSV-HCV Pseudotypes as a Prelude to Recombinant VSV in HCV Immuno-Intervention
o Replication Competent and Defective Recombinant VSV as a Vaccine Strategy to Generate Immune Responses
o Recombinant VSV Vaccine Studies
o VSV Expressing HCV Core, E1 and E2 Structural Proteins as a Potential Vaccine and Immunotherapeutic Strategy against HCV Infection
o Conclusions
o Future Trends
o References
* PDF version of book portionChapter 16 Development of an Infectious HCV Cell Culture System Takaji Wakita and Takanobu Kato
o Introduction
o A Case of Fulminant Hepatitis Associated with HCV
o Sequence Analysis of JFH-1
o Preferential Processing for Core Protein of JFH-1
o Replication Capacity of JFH-1 as a Subgenomic Replicon
o Construction of Full-Length JFH-1 cDNA
o Replication of Full-Length JFH-1 RNA in Huh7 Cells
o Infectivity of Secreted Viral Particles from JFH-1 Transfected Cells
o Neutralization of JFH-1 Infectivity by CD81 Antibody and Patient Sera
o In Vivo Infectivity of JFH-1 Culture Medium
o Permissive Cells for JFH-1 Infection
o Summary and Concluding Remarks
o References
* List of Figures

Copyright © 2006 Horizon Bioscience, an imprint of Horizon Scientific Press